DARU Journal of Pharmaceutical Sciences 2010. 0(1):20-25.

Impact of Black seed (Nigella sativa) extract on bone turnover markers in postmenopausal women with osteoporosis
N Valizadeh, H.R Zakeri, G Amin ansafi, A Shafiee, P Sarkhail, R Heshmat, H Sereshti, B Larijani




Background and the purpose of the study:

Experimental studies have shown that Ns (Nigella sativa) seeds oil can increase bone formation and may have anabolic effects on bone loss. This study was conducted to investigate the beneficial impacts of the oil of Black seeds on bone turnover in osteoporotic postmenopausal women.

Materials and methods: A placebo controlled pilot study was carried out on 15 postmenopausal osteoporotic women of 48-74 years old. In addition to Calcium-D supplements (2 tablets per day) all participants were randomly received Ns extract (3ml, 0.05 ml/kg/day p .o.) or placebo for 3 months. In all subjects hematological tests were performed and hepatic enzymes, BUN, Cr, Ca, P and plasma bone formation and resorption markers including osteocalcin, bone alkaline phosphatase (Bone-ALP) and carboxy terminal cross linked telopeptide (CTX) was determined before and after 12 weeks of treatment.

Results: Twelve participants completed the entire 12 weeks study course of which 5 and 7 women were belonged to Ns and placebo groups respectively. Women in placebo group were significantly older than women in Ns group. There were not significant differences between BMIs, BMD results and plasma levels of bone marker in two groups at the baseline and plasma levels of bone markers between Ns and placebo group at the end of 12 weeks. Alterations from baseline in bone markers levels did not differ significantly between two groups. We did not observe any side effects due to Ns therapy.

Conclusion: In this pilot study similar to the previous trial, we failed to show beneficial impact of Ns extract administration for a short time on bone turnover so we don’t suggest it for medicinal application in the osteoporosis condition. Long time duration studies with larger sample size and usage of a more tolerable dosage forms of Black seeds oil should be emphasized for further clarification of its useful anabolic effects on bone metabolism.


Black seed (Nigella sativa), Bone markers, Osteoporosis,

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