DARU Journal of Pharmaceutical Sciences 2001. 9(3-4):50-57.

Interaction of L-Arginine/Nitric Oxide system with Lead Acetate on secretion of Amylase from isolated rat parotid glands
Abdollahi M, Zadehkabir R, Rahmat Jirdeh N, Dehpour AR


In the present study the effects of lead acetate and/or L-Arginine as a nitric oxide precursor and L-NAME as a nitric oxide synthase inhibitor on the amylase secretion of rat parotid gland lobules were investigated. Lead acetate in doses of 3, 30 and 300 µM significantly (P<0.01) caused a dose-dependent reduction in isoproterenol-stimulated or non-stimulated amylase secretion. When secretion of saliva was not stimulated by beta-adrenergic agonist, L-Arginine (100 µM) significantly (P<0.01) reduced amylase output. L-NAME (100 µM) alone had no significant effect on amylase output but when used with lead acetate prevented (P<0.01) from lead-induced reduction of amylase output. Both L-NAME (100 µM) and L-Arginine (100 µM)) when used alone reduced isoproterenol-stimulated amylase output. Concurrent administration of lead acetate (300 µM)) with either L-Arginine (100 µM) or L-NAME (100 µM) showed a marked positive interaction in reducing the isoproterenol-stimulated secretion of amylase. These findings suggest that nitric oxide plays a role in secretion of amylase from parotid. Different affinity of lead acetate to interact with different nitric oxide synthases might be a reason for different effects on parotid amylase secretion observed in the presence or absence of secretion stimulant.


Parotid, Amylase, L-NAME,

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