Morphological and Phytochemical Investigations on Crataegus curvisepala and Crataegus oxyacantha

Abstract

Several species of the genus Crataegus have been used for treatment of hypertension and certain cardiac disorders. Over than 20 species of this plant is extensively grew in Iran. One of these species is C. curvisepala.
in this study, C.curvisepala was examined botanically and phytochemically in comparison to C, oxyacantha.
Morphological as well as microscopical characteristics of C. curvisepala was examined and some differences distinguished from C. oxyacantha. By means of TLC in comparison to authentic samples,rutin, hyperoside and chlorogenic acid were identified in these plants. By preparative TLC method, rutin is isolated and then purified from these plants. The structure of rutin was determined by the UV-Vis. Techniques in methanol and by addition of the shift reagents and hydrolysis. The quantitative determinations of flavonoids in these plants were also performed by using an UV-Vis. spectroscopy method.