The Effects of nitric oxide synthase inhibitor (L-NAME) on epididymal sperm count, motility, and morphology in varicocelized rat

Abstract

Introduction: Increase in the nitric oxide in the spermatic veins of men by varicocele has been reported. Although Several studies have considered the relationship between varicocele and semen NO concentrations, no study on the effects of nitric oxide synthase inhibitor (L-NAME) on epididymal sperm count, motility and morphology which are important in fertility of the individual has been reported. The aim of study was to evaluate the effects of nitric oxide synthase inhibitor (L-NAME) on epididymal sperm count, motility, and morphology in varicocelized rat.

Methods: Twenty four Wistar male rats divided into four groups. The group A and B underwent a left experimental varicocele (by 20-gauge needle). Group C, underwent a procedure similar to groups A and B without any change on spermatic vein (as sham group). Group D referred to as control. Animals in group A were killed 10 weeks after the operation and both left and right epididymal sperm were counted and their morphology and motility were analyzed. Animals in group B received 10mg/kg L-NAME intraperitoneally daily for ten weeks.

Results: In group A, Sperm count decreased and the morphology changed significantly in comparison with the groups C and D. The sperm morphology in groups A and B showed statistically significant differences (P<0.0001). Sperm motility decreased significantly in the group A in comparison with the groups C and D. Although motility in group A of animals were different in comparison with group B , it was not statistically significant.

Conclusion: These findings suggest that nitric oxide synthase inhibitor (L-NAME) improved sperm count and morphology.