Hyoscyamine 6 b-Hydroxylase Gene Isolation from inVitro Cultured Roots of Hyoscyamus niger l. and Hyoscaymus Tenuicaulis Schonbeck-temesy

Abstract

The H6H gene for hyoscyamine 6b-hydroxylase, which converts hyoscyamine to scopolamine, was isolated from H. niger and H. tenuicaulis. The roots of 14 days sterile seedlings were transferred to a modified liquid B5 medium containing 1mM indolebutyric acid, and after appearance of the lateral roots, subcultured in a free hormone medium. Following a week, the total cellular RNA of the roots was extracted. cDNA of H6H gene was synthetized by RT-PCR, favored gene was amplified by the PCR.