DARU Journal of Pharmaceutical Sciences 2000. 8(1-2):36-41.

IDENTIFICATION AND AMPLIFICATION OF HYOSCYAMINE 60-HYDROXYLASE GENE IN GENOMIC DNA OF IN VITRO CULTURED ROOTS OF HYOSCYAMUS NIGER L. BY POLYMRASE CHAIN REACTION
HASSAN EBRAHIMZADEH, AFSANEH TEIMOORI TAHMINEH LOHRASEBI

Abstract


The H6H gene for hyoscyamine 6B-hydroxylase (H6H), which converts hyoscyamine to scopolamine, was isolated from Hyoscyamus niger. The roots of 14 days sterile seedlings were transferred to a modified liquid B5 medium containing 1 uM indolebutyric acid, and after appearance of the lateral roots, subcultured in a free hormone medium. Following a week, its genomic DNA was extracted and PCR performed. By extraction an accurate DNA and using suitable primers, H6H gene can be isolated completely.

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