DARU Journal of Pharmaceutical Sciences43MECHANISM OF THE OXIDATION OF HEMOGLOBIN BY COPPER (II) COMPLXES1623M. BAYATI20151006An outer sphere electron transfer mechanism by which human hemoglobin reduces the complexes of copper(II) and, in turn, is oxidized to methemoglobin has been characterized. We have found that the rate of oxidation of hemoglobin is a function of pH, temperature, concentration of copper(II), and the environment of the hemoglobin. Prior to oxidation, copper(II) complex binds to specific sites on the surface of the protein by losing one or more of its ligands, forming a ternary complex. This process is followed by electron transfer between the Cu(II) and Fe(H) with the Cu(II)-deoxyhemoglobin being the active intermediate. The dominant factors which govern the rate of oxidation of hemoglobin by coppcr(I I) complexes seem to be the stability constant of the Cu(II) complexes and the overall redox potential of the ternary complex.https://daru.tums.ac.ir/index.php/daru/article/view/46https://daru.tums.ac.ir/index.php/daru/article/download/46/46

DARU Journal of Pharmaceutical Sciences43ESTIMATION OF SALICIN IN BARKS AND LEAVES OF SALIX SPECIES BY A TLC-SPECTROPHOTOMETRIC METHOD815S. AfsharypourH. Kazeroony20151006Barks and leaves of most Salix species yield a medicinally acLivc glycoside called salicin. To determine the quality of herbal drugs obtained from Salix plants, salicin content of stem-barks and leaves of Salix acmophylla Boiss., Salix aegyptica L., Salix alba L., Salix babylonica L., Salix carmanica Bornm, and Salix excclsa Gmelin, which are growing in Isfahan, was determined.Two spectrophotometric methods were used in this research. In one method, which is proposed in this work and which is called combined TLC-spectrophotometric method, salicin was separated by using Thin Layer Chromatography technique (TLC), then the intact glycoside was determined spectrophotometrically.In the other method, called combined TLC-colorimetric method, freshly prepared Erdmann's reagent was used to produce a colored complex with the separated salicin. Then the intensity of the produced color was determined.The results of both applied methods proved that:1) The proposed TLC-spectrophotometric method is more sensitive than the LC-colorimetric one.2) Stem-barks of all examined species contain more salicin compared to the salicin content of leaves of the same plants.3) Stem-barks of Salix carmanica Bomm contain the highest quantity of salicin when compared to the salicin content of stem-barks and leaves of other five species examined in this work.https://daru.tums.ac.ir/index.php/daru/article/view/47https://daru.tums.ac.ir/index.php/daru/article/download/47/47

DARU Journal of Pharmaceutical Sciences43ANALYSIS OF BETAMETHASONE DISODIUM PHOSPHATE INJECTION AND OPHTHALMIC SOLUTION BY HPLC, KINETIC INTERPRETATION AND DETERMINATION OF SHELF LIFE17A. GhanbarpourM.Farahani - Nik M.Amini20151006Corticosteroids are widely used in therapeutics in different formulations. Betamethasone disodium phosphate is one of the most soluble of the adrenocorticosteroidal agents. It is therefore very suitable for intravenous use and particularly for ophthalmic formulations.Acceleration method is used to determine expiration date of aqueous formulations manufactured in Iran.https://daru.tums.ac.ir/index.php/daru/article/view/48https://daru.tums.ac.ir/index.php/daru/article/download/48/48