DARU Journal of Pharmaceutical Sciences41-2Rutin from Ruta graveolens L112Dj. AfsharA. Delazar20151006Quantitative determination of rutin in Ruta Graveolens, L. which is easily cultivated in Iran, is necessary for its economic importance, diversity of specialities and their pharmacological activity in blood vessels disorders. For this purpose, the plant was cultivated in botanical garden of Tabriz University of Medical Sciences and rutin was determinated at different stages of plant growth. The extraction was done from the crude plants by ethanol, boiling water and hydroalcoholic solvents. Rutin was subsequently purified by crystalization.Structure elucidation was confirmed by the U.V spectrum in the presence of shift-producing reagents, and TLC by using different systems, HPLC and HPTLC in the presence of standard.Sugars and their sequence of bonding to genine were known by acidic hydrolysis, sequential hydrolysis and TLC in the presence of standards. For quantitative determinations of rutin two methods were selected i.e. HPLC and colorimetery. In the colorimetrey method, cyanidin reaction was used for color producing and the absorbances of other compounds of extract were eliminated in coincidence with rutin absorption.. In the HPLC method, separation was done on CN- column with mobile phase including dioxan/phosphate bnffer (pH=2.6); 2:8. In the end the results of two methods were compared.https://daru.tums.ac.ir/index.php/daru/article/view/41https://daru.tums.ac.ir/index.php/daru/article/download/41/41

DARU Journal of Pharmaceutical Sciences41-2CONSTITUENTS OF THE ESSENTIAL OIL OF "MENTHA MOZAFFARIANI JAMZAD"1325DjA.RustaiyanM.M. SaberiA.R. Jassbi20151006The genus Mont ha is widely distributed in Iranian territory and comprises several species reported in folk medicine as having biological activity (1). Mentha mozaffariani Jamzad, growing in the wild in southern Iran, near Bandar-Abass (40 km. north of the Persian Gulf), contains an essential oil, the exact composition of which has not yet, as far as we know, been reported. Spectrometric scanning (IR and H-NMR) of the crude oils indicated their terpenic nature.The aerial parts and flowers of this plant were investigated by means of gas chromatography, mass spectrometry and high-resolution H-NMR of the main compounds. The composition of the volatile mixtures was determined by comparison with data from the literature, and databases of the retention times and Kovats indices.The compounds most frequently found in the oils studied are:-caryophyllene, a - Humulene, calamenene and piperitone epoxide. Among hydroxylated terpenes, a -terpineol, 4-terpineol, linalool and borneol were found.https://daru.tums.ac.ir/index.php/daru/article/view/42https://daru.tums.ac.ir/index.php/daru/article/download/42/42

DARU Journal of Pharmaceutical Sciences41-2Study of Afecting agents on physical Properties of Aspirin- Tableted Microcapsules2738DJ. Farid - N. BlourchianNokhodchi20151006IR disc preparation device at different compression forces.lt was shown t constant compression force, the particle size of microcapsules has no influence on tablet thickness but the tablet hardness was in direct relation with particle size. The tablets prepared with microcapsules and P.V.P. were thicker than thoseobtained from microcapsules on its own and mixture of microcapsules and lactose. The hardness of tablets2 prepared with microcapsules alone remained unchanged at compression forces superior to 100 kg / cm .Whereas the hardness of tablets containing mixture of lactose and microcapsules increased as the compression force went up. Finally, the hardness of tablets obtained from P.V.P and microcapsules decrease at high compression forces.These considerations nature of excipient and compression force modify considerably the hardness, thickness and porosity of tablets.https://daru.tums.ac.ir/index.php/daru/article/view/43https://daru.tums.ac.ir/index.php/daru/article/download/43/43

DARU Journal of Pharmaceutical Sciences41-2Chemical Analysis of Essential oil of "Artemisia haussknechtii Boiss" by GC and GC/ MS3949A. Nassir- Ahraadi . A. RustaiyanA.R. Jassbi20151006The composition of the essential oil from the leaves and flowers of "Artemisia haussknechtii Boiss growing wild in the north-west of Iran, was investigated by GC and GC/MS.The main components of the volatile oil were 1,8 - cineol (16.5%), camphor (14.1%). artemisia ketone (10.5%), fragranol (9.0%), Yomogi alcohol (7.5%) and B- pinene (5.4%). The total contribution of these compounds to the oil amounted to 63.0%.Monoterpens and sesquiterpenes represent 90.08% and 1.52% of the oil respectively. Of the twenty oxygen-containing monoterpenes which made up a fairly large fraction of the terpenoid composition, the predominant components were 1,8 - cineole and camphor.https://daru.tums.ac.ir/index.php/daru/article/view/44https://daru.tums.ac.ir/index.php/daru/article/download/44/44

DARU Journal of Pharmaceutical Sciences41-2DETERMINATION OF ENDOTOXIN IN SMALL-VOLUME PARENTERALS (SVPs): A COMPARISON OF LAL GEL-CLOT METHOD WITH U.S.P. RABBIT PYROGEN TEST115Morteza Rafiee-Tehrani M.H. Djamshidi20151006The simplicity and sensitivity of the Limulus Amebocyte Lysate (LAL) gel-clot method in detection of endotoxin in Small Volume Parenterals (SVPs) were studied and compared with rabbit pyrogen test.All formulations were artificially spiked with 0.25, 0.5 and 5EU/ml of Escherichia coli 0111:B4 endotoxin (Iambda=0.5EU/ml). The Threshold Pyrogenic Dose (TPD) for E.coli 0111 :B4 endotoxin was found to be more than lng per kg of body weight (0.5EU/ml). However, the sensitivity of LAL test was found to be 0.25 Eu/ml for water for injection and 0.5 EU/ml for other formulations. The observed inhibitory effect of electrolyte containing solutions was deleted by some degree of dilution prior to test. However, none of the samples showed inhibitory at the Maximum Valid Dilution (MVD). The U.S.P. rabbit pyrogen test was insignificant for all the solutions tested. These results suggest, LAL gel-clot test to be useful for determination of bacterial endotoxin as final release test in SVPs. A comparison of the pyrogenicity assays also indicates, that LAL is more sensitive and simple and less time-consuming than the conventional rabbit test.https://daru.tums.ac.ir/index.php/daru/article/view/45https://daru.tums.ac.ir/index.php/daru/article/download/45/45