DARU Journal of Pharmaceutical Sciences31Determintation of sulfor containing glucosides in Brassica nepus L. and Sinappis alba L. tissue cultures17Suleiman Afsharypuor20151006In this study, hypocotyls of 5 day old seedlings of Brassica nepus L. and Sinappis alba L.. were explanted onto Murashige and Skoog's revised Tobaco Medium(l)containing different concentrations of suitable plant growth regulators (a cytokinin and an auxin). In addition to the plant growth regulators, potassium sulfate(in a concentration of 3 m. eq./liter) was added to some solid and liquid media in an attempt to raise available sulfate levels, to other medium, L - B- Phenylalanine was added (in a concentration of 30 m. eq./liter) to act possibly as a precursor for the biosynthesis of sulfor containing glucoside (glucosinolate). Suspension cell cultures were initiated from callus and grown in the same medium without agar. Calli which were harvested at 20 weeks and cells at 6 weeks after subculture, were dried and examined by capillary GC for the presences of sulfor containing glucosides. The obtained results indicated the absence of such glucosides in the cells and calli.https://daru.tums.ac.ir/index.php/daru/article/view/34https://daru.tums.ac.ir/index.php/daru/article/download/34/34

DARU Journal of Pharmaceutical Sciences31Evaluation of absorption and pharmacokinetic parameters of various injectable products of gentamicin in rabbits917M.Bijanzadeh M.MahmoudianM.Farzin MothammedA.Khosravy20151006Gentamicin (GM) is an aminoglycoside antibiotic with a narrow therapeutic range and severe adverse effects. The relation between its serum concentration and toxicity and neprotixicity have been estabilished. It has been recommonded that its peak serum concentrations should not be exceed 12 to 15 ug/ml. For these reseans this study was undertaken to evaluate the bioavailability and pharmacokinetics characteristics of generic gentamicin l0 mg/ml (GM/G) - compared to g entamicin 40mg/ml (GM/B). Eight adult and healthy rabbits weighed 1.5. 2. 75 Kg. were given GM as a single IM injection (2. 5 mg/kg). Blood samples were collected from the ear vein and serum was separated and kept frozen prior to assay. Serum concentrations of GM were determined using enzyme multiple immunoassay technigue EMIT (syva). Pharmacokinetics parameters were calculated from the serum concentrations data with the aid of computer program. It has been found that the pharmacokinetics of GM follows a two compartmental model, and:1 - Tl 2 elim.elimination half life, is 1. 57 h, for GM/G and 1.13 h. for GM/B. tl/2 dis, distribution halflife, is 0. 86 for GM/G and 0. 45h . for GM/B.2 - Cp max' Peak plasm level is 7.83 mg/L for Gm/G and 8.38 mg/L for. GM/B.3 - Tmax time to reach peak plasm level is 0.37 . for GM/G and 0. 4oh . for GM/B.4 - Auc 4, Area under concentration - time curve to 4 hour, and Auc inf. Area under concentration. - timecurve to infnity are 10. 94, 11. 32 mgml/h for GM/G and 10.17. 10.48 mgml/h for GM/B .Statistical analysis T. Test, has shown Theve is no significat difference between These two products andtherefore, These products are bioequivalent.https://daru.tums.ac.ir/index.php/daru/article/view/35https://daru.tums.ac.ir/index.php/daru/article/download/35/35

DARU Journal of Pharmaceutical Sciences31A COMPARATIVE STUDY ON MACROSSOPIC, MICROSCOPIC AND PHYTOCHEMICAL PROPERTIES OF EUCALYPTUS SPEJES, SPEJES, CULTIVATED IN IRAN, WITH STANDARD SPECIES2434Samsam shariat sadeghi-aliabadi20151006The aim of this study is to compare the volatile oil of staendard species of Eucalyptus with those species cultivated in Iran. The investigation was carried out as bellow:A) The seeds of elven species were supplied from Germany and cultivated in Research Center of Agriculture of Isfahan and then extracted for volatile oil and cineole.B) Besides, we supplied 2 species from Isfahan, 7 species from Fars province, 9 from Khuzestan province, one from Tehran and one from Hormozgan, province.C) We extracted total volatile oil of 28 E. species, then injected to G. G. column and the results were as follow:1 - In the following species : E. saligna, E. loxophleba and E. Stratycalyx from Esfahan province. E.loxophleba, E.camaldulensis, E.stratycalyx, intertexta and E. caleicultrix from Farsprovince. E. Stratycalyx, E. OCCidsntalis, E. gillii and E. sargentii from khozestan prcvince. E. globulus from Tehran and E. camaldulensis from Hormozgan province. The amount of essential oil extracted from the mentioned species was in accord with the standard of pharmacopial level. But the extracted oil from others was not as standard levl.2 - E. gillii, E. camaldulensis, E. sargentii and OCCidentalis from khouzestan and the species ofE.intertexta, E. camaldulensis, E. lansdownean from fars and the species of E. camaldulensis, E. saligna from Esfahan and the species of E. globulus from Tehran, the amount of measured cineol was in accordance the standard pharmacopeial level.3 - Some of the species studied such as : were qualified for the amount of Alpha and Beta pinen.https://daru.tums.ac.ir/index.php/daru/article/view/36https://daru.tums.ac.ir/index.php/daru/article/download/36/36

DARU Journal of Pharmaceutical Sciences31EFFECT OF ORAL INSULIN IN BLOOP G1UCOSE CONCENTRATION3544DJ. FARIDM.R. HASHEMISH. P. TABRIZlM. ADRANGUlN.Namvar20151006Gastrointestinal tract can not be used as a route for oral administration of polypeptid hormones becauseof their enzymatic degradation.Degradation of these macromoleculcs in acidic and alkaline conditions determines the need for usingprotective delivery systems.In this research microcmulsions were used for protection of insulin against proteolytic enzymesofgastrointestinal tract. Cholestrol and phospholipids of egg yolk have been used as lipid phase as lipid phaseand Lecithin as surfactant.Insulin Regular was used as aqueous phase, being entrapped with lipidic phase in W/O manner. Malerabbits with body weight of about 1-1.5 KG were accomplished and oral insulin was force fed to them.Blood collection has been carried out from heart every 15 minutes after oral administration.Reduction in blood glucose level indicates the well being protection of insulin and absorbtion of it throughepithelium of small intestine. Increasing of glucose level in placebo demonstrates that endogenousinsulin has not been responsible for serum glucose reduction.This experiment suggests that microemulsions formed with egg Yolk compounds have the ability to be analternate for parenteral administration of insulin and other chemicals sensitive to enzymatic degradation, inhuman.https://daru.tums.ac.ir/index.php/daru/article/view/37https://daru.tums.ac.ir/index.php/daru/article/download/37/37

DARU Journal of Pharmaceutical Sciences31A Phytoch emical study on silybum marianum L. of Iran4554N. GhassemiF. Moattar20151006Considering the great importance of silybum marianum L. for treatment of hepatic diseases and since it has been remained unknown in Iran, the standards fruits ofthis plant were planted in pharmaceutical plants research center of Isfahan. The available species in Iranwas collected to compare the macroscopy and microscopy properties of the both species and the percentageof their fruits silymarin and Quercetine.The fruits were analyzed by chromatography and then the precentage of their "Silymarin" were determinedby spectrophotometry.The results of analysis showed that the both species have "Silybin' and Quercetin", and the precentage ofsilymarin in the silybum marianum collected from Iran is more than the standard.https://daru.tums.ac.ir/index.php/daru/article/view/38https://daru.tums.ac.ir/index.php/daru/article/download/38/38

DARU Journal of Pharmaceutical Sciences31Screening of Iranian plants for Antimicrobial Activity III5562M. H. SALEHI SURMAGHIGH. AMIN20151006Third part of Iranian plants were screened for antimivrobial activity. Of 109 species representing 44 families, 3 were active against Klebsiella pneumo niae, 1 active against prOteus vulgaris, 1 active against Shigella sonei, 2 active against Vibrio cholerae, l active against Escherichia coli, 6 active against Staphylococcus aureus , 21 active against Bacillus anthracis and 2 active against Salmonella paratyphi AThe previous paper of this series recorded the results of antimicrobial activity of 267 species of Iranian plants (1-2). The present paper reports the results of antimicrobial activity of 109 species repersenting 44 families collected from different parts of Iran. Methanolic extracts of the plants were tested for antimicrobial activity against 9 organism (Table 1), and the results of antimicrobial activity are presented (Table 2).https://daru.tums.ac.ir/index.php/daru/article/view/39https://daru.tums.ac.ir/index.php/daru/article/download/39/39

DARU Journal of Pharmaceutical Sciences31Volatile Constituents of Zhumaria Majdae6369YazdanparstR.SicariS20151006Capillary gas chromatography mass spectrometry (GC- MS) analyses of a sample of essential oil of zhumaria Linalool ned by simple water distillation of the pulverized air - dired leaves and flowers of the plant indicated that Linalool and comphor are the two major constituents of the volatile oil. Sylvestrene , y -terpinene, a- Pinene, b - carene, camphene, and Epiborneol constitute the other main components of the essential oil. The GC - MS chromatogram indicated the presence of more than fifty - components in the oil, most of them were present in trace amounts. In this study, the chemical structures of twenty of these consti tuents were elucidated using GC - MS analysis.https://daru.tums.ac.ir/index.php/daru/article/view/40https://daru.tums.ac.ir/index.php/daru/article/download/40/40