DARU Journal of Pharmaceutical Sciences22-3Quantitative determination of Glycyrrhizine and Glycyrrhetic acid in roots of Liquorice by HPLC229236DJ. Afshar A-Delazar20151006Most of the methods of determination of Glycyrrhizine in the roots of Liquorice are non - specific and relying on indirect methods. The results obtained from these methods are usually unreal and unreliable. In this paper Glycyrrhizine iv various Liquorice roots was estimated directly by HFLC without hydrolysing Glycyrrhizine to its aglycone. Hence the existing problems that considerably change results are eliminated. In this method, Glycyrrhizine was separated from other components of total extract using reverse phase high performance liquid chromathography and the results of determinations have been satisfactory and reproducible. The Glycyrrhizine content was 2.15 -5.4 percent in the various liquorice roots.Also suitable condition is reported for HPLC of Glycyrrhetic acid in hydrolyzed total extract. Therefore Glycyrrhizine was also determinated by indirect method. This method is based on hydrolysing Glycyrrhizine and estimating Glycyrrhetic acid . Results of direct method are compared with this method.https://daru.tums.ac.ir/index.php/daru/article/view/28https://daru.tums.ac.ir/index.php/daru/article/download/28/28

DARU Journal of Pharmaceutical Sciences22-3Introducing Aztreonam The First Monobactam Antibiotic, A Suitable Substitution for the Aminoglycosides238248A. Jahanshahi M.Khajeh - Karamadeni S. Fazli Bazaz20151006Aztreonam (Azactam) for injection, squibb) is the first member of a new and unique class of beta - lactam antibiotics designated by researchers at the Squibb Institute for Medical Research as monobactams (monocyclic bacterially produced beta - lactam antibiotics).In this research, for the first time, antimicrobial spectrum of aztrenoam was determined by Disk - Agar Diffusion (250 spp.) and Macrodilution Broth Methods (150 Spp.)We also compared this antibiotic with two routine aminoglycoside antibiotics (Amikacin, Gentamicin) in Iran. The most active antibiotic in our study was aztreonam which had MIC50 & MIC90 of 4 & 32 ^g/ml specifically against Pseudomonas aeruginosa.This rate for the other aerobic gram - negative bacteria (E. coli, Kleb. pneumoniae, Proteus mirabilis, enterobacter spp., Shigella Spp. and Salmonella Spp.) was less than 0.5 & 4 g/ml respectively.Aztreonam's MIC90 for kleb pneumoniae was 8/jg/mI our results were Correlated to the other studiesAll aerobic gram - negative bacteria has been obtained from the Qhaem's Medical Center in Mashhad - IRAN.The results of Disk - Agar Diffusion Method determines that all bacteria were 100% susceptible against aztreonam except Pseudomonas aeruginosa with 83% susceptibility.https://daru.tums.ac.ir/index.php/daru/article/view/29https://daru.tums.ac.ir/index.php/daru/article/download/29/29

DARU Journal of Pharmaceutical Sciences22-32D 1H -13C Heteronuclear Shift Correlation Of 2a - Hydroxy Aiantolactone From Pulicaria Undulata C.A. Mey250259A. RustaiyanZ. Habibi20151006We have reported recently the isolation and characterization of several sesquiterpene lactones from Pulicaria undulata (1).The lactones were isolated from an Et20 - Petrol (1:3) fraction by different chromatographic techniques including HPLC (RP 8, MeOH - H2O, 13:7).In this way three eudesmanolides 1 - 3, a guaianolide 4, a nor -guaianolide 5, as well as the pseudoguaianolide 6 and the xanthanolide 7 were isolated. One of the eudesmanolides (2a - hydroxy aiantolactone), 1, was present as the main component.Such lactones being known as biologically active substances, we have decided to describe for the first time a detailed interpretation of proton, 1H -NMR, 13C - NMR and 2D lH -13C - heteronuclear shift correlation spectra of 2a - hydroxy aiantolactone. The stereochemistry of C - 2 , C - 7 and C - 8 was determined by the NOESY experiments, H - 7 and H - 8 are in the a configuration and H - 2 is in the b configuration.https://daru.tums.ac.ir/index.php/daru/article/view/30https://daru.tums.ac.ir/index.php/daru/article/download/30/30

DARU Journal of Pharmaceutical Sciences22-3Determination of impurities in Nifedipine, Clofibrate, Atenolol, timolol, propranolol and Ranitidne by HPLC261272A. ShafieeF. Shojaie20151006In this work the impurities of the title compounds were investigated by HPLC. the samples were collected from drug stores and the Bulk Chemicals were obtained from manufacturers. The samples were dissolved in suitable solvents before analysis. The Detector was UV and the Cloumn was bondapack Cjg(30 cmx3.9 mm). In the case of timolol the colum was microbondapack CN.The impurity in nifedipine was 2, 6 - dimethyl -3,5-dicarbomethoxy - 4-Nitrosophenylpyridine; in clofibrate was para - chlorophenol; in atenollol was p - hydroxyphenylacetamide; in ranitidine was ranitidine - N-oxide, 2,2-dimethylthiol - nitroethene and two unknown compounds. In timolol and propranolol one impurity was detected. The amount of impurities in all compounds were in acceptable range.https://daru.tums.ac.ir/index.php/daru/article/view/31https://daru.tums.ac.ir/index.php/daru/article/download/31/31

DARU Journal of Pharmaceutical Sciences22-3physical properties of some powder's formulations273280M. GharaviA. Hossini20151006It is well known that there is a sound correlation between moisture content of a powder and physical properties such as flow, cohesivness, porosity, packing, strength of van der waals forces and electrical charges. Experiments were carried out with series of ten powder formulations (Contain : Kaolin, talc, starch, zinc oxide,zinc stearate and sodium bicarbonate). Relationship of their properties to the samples are measured and discussed for samples one to five quantity of kaolin and talc flactuate between 5% to 75% for formulations 6 to 10, starch and kaolin have the same flactuations as first ones. Water was added to the samples in amount of 0% , 1% , 2% ... 10%, according to the plotes of bulk density versus number of taps at different water content, some samples are in different position from the others. Porosity for formulation 1 to 10 decrease as moisture is increased, but bulk density increased except for 0% and 10% moisture for samples 4,5,9 and 10. Repose angle for formulations and 6 contain 2% moisture indicated that electrostatic charge quite decreased, therefore flowability of these samples increased. Furthermore it is proven that samples contain kaolin is much more hygroscopic than formulations contain talc and starch.https://daru.tums.ac.ir/index.php/daru/article/view/32https://daru.tums.ac.ir/index.php/daru/article/download/32/32

DARU Journal of Pharmaceutical Sciences22-3Survey of Iranian Plants For Saponins Alkaloids Flavonoids And Tannins. IV111M.H. Salehi SurmaghiY. Aynehchi GH. AminZ. Mahmoodi20151006A total of 149 plant extracts representing 49 different families has been screened for saponins, flavonoids, tannins and alkalodids. positive tests obtained were 145 (97) for saponins 74 (50%) for alkaloids 16 (51%) for fiavonoisds and 60 (40%) for tannins.https://daru.tums.ac.ir/index.php/daru/article/view/33https://daru.tums.ac.ir/index.php/daru/article/download/33/33