DARU Journal of Pharmaceutical Sciences93-4Pattern secretion of matrix Metalloproteinases and their biological tissue inhibitors by human glomerular mesangial cells in culture18"Hosseini RHampel GJung K "20151006The glomerular mesangial cells (GMC) play a central role in the synthesis and turnover of the glomerular mesangial matrix. The breakdown of the matrix likely depends on the balance between of a variety of proteinases including matrix metalloproteinases and their biological inhibitors secreted by the GMC, and any disturbance in the balance may result in appearance of various pathological states such as glomerulosclerosis. We therefore studied pattern secretion of matrix metalloproteinases (MMPs), MMP-1, MMP-2, MMP-3, MMP-9 and their biological tissue inhibitor of matrix metalloproteinases (TIMPs), TIMP-1 and TIMP-2 by cultured human GMC. We also measured MMP-1/TIMP-1 complex level in the cell culture supernatants. For this purpose, the GMC were incubated under serum-free conditions with medium (RPMI-1640) alone or in combination with TNF-α (30 ng/ml) or phorbol myristate acetate (PMA) (50 ng/ml) for exactly 24, 48 and 72 hours. The above parameters were assayed by established ELISA techniques. Our results showed that the lowest and largest secretions were related to MMP-9 and MMP-2, respectively. The results indicated that the MMPs and TIMPs secretion were increased by TNF-α (MMP-1, MMP-2, TIMP-1 and TIMP-2) and PMA (MMP-2, TIMP-1 and TIMP-2), significantly (Phttps://daru.tums.ac.ir/index.php/daru/article/view/121https://daru.tums.ac.ir/index.php/daru/article/download/121/121DARU Journal of Pharmaceutical Sciences93-4"Comparison of biodistribution of ¹¹¹In-Tropolone leukocytes and 125I-human nonspecific polyclonal IgG in normal and induced inflammation mice for detection of inflammation "917Shah Hosseini SHadizad TBabaei MHNajafi R20151006Human nonspecific polyclonal IgG and granulocytes, which accumulate in inflammation foci, were radiolabeled with 125I and ¹¹¹In-Tropolone, respectively. Biodistribution of these two radiolabels was assessed in normal and inflammation-induced mice. 125I-IgG showed better localization to the inflammated areas. Blood levels with ¹¹¹In-Tropolone leukocytes were lower at all time points. In addition, the inflammatory thigh-to-blood ratios showed an improvement, whereas the ratios of inflammatory thigh-to-other normal tissues were higher for 125I-IgG than ¹¹¹In-Tropolone leukocytes. In conclusion, labeled IgG due to better localization in inflammated sites and higher target-to-background ratios is more suitable agent than labeled leukocytes for immunoscintigraphy of inflammation.https://daru.tums.ac.ir/index.php/daru/article/view/122https://daru.tums.ac.ir/index.php/daru/article/download/122/122DARU Journal of Pharmaceutical Sciences93-4Mutagenesis of Leuconostoc Mesenteroides and selection of Dextransucrase hyperproducing strains1823"Kamal FSamadi NMazaheri Assadi MMoazami NFazeli MR "20151006Dextransucrase of Leuconostoc mesenteroides PTCC 1059 is an enzyme of industrial and medical interest that catalyzes the synthesis of a soluble dextran from sucrose. The mutant strains of Leuconostoc mesenteroides PTCC 1059 hyperproducing for dextransucrase were isolated after UV irradiation and treatment with ethyl methane sulfonate. The enzyme activity of one mutant strain, the 1059M5E4, was about 2.5-fold higher than that of the wild type, while its cell growth was relatively lower. The 1059M5E4 dextransucrase produced the same type of dextran as well as the wild type but showed higher thermal stability. These properties may be interesting for using this strain in enzymatic production of dextran.https://daru.tums.ac.ir/index.php/daru/article/view/123https://daru.tums.ac.ir/index.php/daru/article/download/123/123DARU Journal of Pharmaceutical Sciences93-4Alteration of Pentylenetetrazol-induced kindling parameters by prenatal chronic Lead exposure in rats2427Kebriyaei Zadeh AMassihaye Akbar RSoltani Nejad K20151006The effect of prenatal chronic lead exposure on pentylenetetrazol (PTZ)-induced kindling parameters (seizure index, seizure latency and seizure stage) in rats was studied. Adult female rats with a weight range of 140-180 g were selected and pretreated with lead acetate (0.05% w/v) orally, 25 days prior to mating. The control group was given distilled water containing sodium acetate solution (0.05% w/v). After delivery, treatment was ceased, and after lactation, male neonates were separated from the females in both groups. After maturation of male rats, the PTZ-kindling was induced by daily interapritoneally injection of PTZ (30 mg/kg). Kindling parameters in the control and treated groups were determined. The results indicated that animals with prenatal lead exposure have full kindling state with 9-19 (16.87±1.54) injections, whereas this value for control group was 12-23 (18.62±1.48) injections. The seizure latency for the treated group was lower (Phttps://daru.tums.ac.ir/index.php/daru/article/view/124https://daru.tums.ac.ir/index.php/daru/article/download/124/124DARU Journal of Pharmaceutical Sciences93-4"Composition of the essential oil of Pycnocycla Spinosa Decne.ex.Boiss. from Isfahan "2829Asghari Gh.RHoushfar Gh.AMahmoudi Z20151006The hydrodistilled aerial parts oil of Pycnocycla spinosa var. spinosa was analyzed by GC and GC/MS. Thirty three components were identified. Geranyl isovalerate (14.9%), caryophyllene oxide (10.6%), α-eudesmol (9.2%) ß-citronellol (7.2%), elemicin (6.8%), r-cymene (4.7%), citronellyl acetate (4.3%), and α-cadinol (3.3%) were found as major components.https://daru.tums.ac.ir/index.php/daru/article/view/125https://daru.tums.ac.ir/index.php/daru/article/download/125/125DARU Journal of Pharmaceutical Sciences93-4"Involvement of metabolic reactive intermediate Cr (IV) in Chromium (VI) cytotoxic effects "3036Pourahmad JO’Brien PJ20151006Addition of Cr VI (dichromate) to isolated rat hepatocytes results in rapid glutathione oxidation, reactive oxygen species (ROS) formation, lipid peroxidation, decreased mitochondrial membrane potential and lysosomal membrane rupture before hepatocyte lysis occurred. Cytotoxicity was prevented by ROS scavengers, antioxidants, and glutamine (ATP generator). Hepatocyte dichlorofluorescin oxidation to dichlorofluorescein (DCF) to determine ROS formation was inhibited by mannitol (a hydroxyl radical scavenger) or butylated hydroxyanisole and butylated hydroxytoluene (antioxidants). The Cr VI reductive mechanism required for toxicity is not known. Cytochrome P450 inhibitors, Particularly CYP 2E1 inhibitors, but not inhibitors of DT diaphorase or glutathione reductase also prevented cytotoxicity. This suggests that P450 reductase and/or reduced cytochrome P450 contributes to Cr VI reduction to Cr IV. Glutathione depleted hepatocytes were resistant to Cr (VI) toxicity and much less dichlorofluorescin oxidation occurred. Reduction of dichromate by glutathione or cysteine in vitro was also accompanied by oxygen uptake and was inhibited by Mn II (a Cr IV reductant). Cr VI induced cytotoxicity and ROS formation was also inhibited by Mn II, which suggests that, Cr IV and Cr IV GSH mediate "ROS" formation in isolated hepatocytes. In conclusion Cr VI cytotoxicity is associated with mitochondrial/lysosomal toxicity by the metabolic reactive intermediate Cr IV and “ROS”.https://daru.tums.ac.ir/index.php/daru/article/view/126https://daru.tums.ac.ir/index.php/daru/article/download/126/126DARU Journal of Pharmaceutical Sciences93-4Antibacterial effect of Glycyrrhetinic Acid on 55 hospital strains of Staphylococcus Aureus and 32 Actinobacillus Actinomycetemcomitans3739Salari MHEshraghi SNoroozi M20151006Glycyrrhetinic acid is a major component of a traditional plant called Licorice. This substance has been found to have some pharmacological properties including anti-inflammatory, anti-viral, anti-bacterial, anti-fungal, anti-allergic, anti-carcinogenic and anti-peptic-ulcer. Glycyrrhetinic acid also affects against some parasites such as Trichomonas vaginalis. In this study, 55 hospital strains of Staphylococcus aureus and 32 Actinobacillus actinomycetemcomitans, were isolated from patient’s specimens by culture method. Antibacterial activities of glycyrrhetinic acid against those microorganisms were investigated by determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. The MIC for S. aureus and A. actinomycetemcomitans were 64 and 8 µg/ml respectively. The MBC for S. aureus and A. actinomycetemcomitans were 64 and 16 µg/ml respectively. It is concluded that Glycyrrhetinic acid is effective against Staphylococcus aureus and Actinobacillus actinomycetemcomitans in appropriate concentrations.https://daru.tums.ac.ir/index.php/daru/article/view/127https://daru.tums.ac.ir/index.php/daru/article/download/127/127DARU Journal of Pharmaceutical Sciences93-4"Synthesis and smooth muscle Calcium channel antagonist effect of Alkyl, Aminoalkyl 1,4-Dihydro-2,6-Dimethyl-4-Nitroimidazole-3,5 Pyridine Dicarboxylates "4045Miri RDehpour ARAzimi MShafiei A20151006The discovery that 1,4-dihydropyridine (DHP) class of calcium channel antagonist inhibits the Ca+² influx represented a major therapeutic advance in the treatment of cardiovascular diseases such as hypertension, angina pectoris and other spastic smooth muscle disorders. A novel class of calcium channel antagonist of flunarizine containing arylpiperazinyl moiety has recently been reported. It was therefore of interest to determine the effect that selected C-3 substituents contained amino alkyl and arylpiperazine, in conjunction with a C-4 1-methyl-5-nitro-2-imidazolyl substituents on calcium channel antagonist activity. The unsymmetrical analogues were prepared by a procedure reported by Meyer in which 1-methyl-5-nitro-imidazol-2-carboxaldehyde was reacted with acetoacetic esters and alkyl 3-aminocrotonate. In vitro calcium channel antagonist activities were determined by the use of high K+ contraction of guinea pig ileal longitudinal smooth muscle. All compounds exhibited comparable calcium channel antagonist activity (IC50=10^-9 to 10^-11 M) against reference drug nifedipine (IC50=2.75±0.36 x 10^-10 M).https://daru.tums.ac.ir/index.php/daru/article/view/128https://daru.tums.ac.ir/index.php/daru/article/download/128/128DARU Journal of Pharmaceutical Sciences93-4Design and evaluation of a new dry powder inhaler4649"Rouholamini Najafabadi AHGilani KHozan AA "20151006Three versions of a new dry powder inhaler (DPI), RG-haler, were designed using two kinds of grid inserts. Salbutamol sulfate/lactose blend (Ventolin Rotacaps®) was selected as a model formulation to analyze the performance of all inhalers and compare their efficiency with three marketed devices (Rotahaler®, Spinhaler® and ISF inhalator®) using the twin impinger (TI). Deposition of the drug in device was significantly (Phttps://daru.tums.ac.ir/index.php/daru/article/view/129https://daru.tums.ac.ir/index.php/daru/article/download/129/129DARU Journal of Pharmaceutical Sciences93-4Interaction of L-Arginine/Nitric Oxide system with Lead Acetate on secretion of Amylase from isolated rat parotid glands5057Abdollahi MZadehkabir RRahmat Jirdeh NDehpour AR20151006In the present study the effects of lead acetate and/or L-Arginine as a nitric oxide precursor and L-NAME as a nitric oxide synthase inhibitor on the amylase secretion of rat parotid gland lobules were investigated. Lead acetate in doses of 3, 30 and 300 µM significantly (Phttps://daru.tums.ac.ir/index.php/daru/article/view/130https://daru.tums.ac.ir/index.php/daru/article/download/130/130