DARU Journal of Pharmaceutical Sciences74HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR DETERMINATION OF NIFEDIPINE IN HUMAN PLASMA14MOHAMMAD ABDOLLAHIMORTEZA PIRALlMUSTAFA KARIMfMASHAALLAH SHAHRIARIAN ABBAS SHAFIEE20151006A relatively simple normal phase high performance liquid chromatography (HPLC) method was modified for determination of nifedipine in human plasma. The method is based on ultraviolet detection at 235 nm and acidic plasma extraction by a mixture of dichioromethane (30%) and n-hexane (70%) using nimodipine as an internal standard. The system was stabilized with the use of n-hexane (80%), chloroform (17%) and methanol (3%) as mobile phase. The assay was linear up to at least 120 ng/ml of nifedipine in plasma. The limit of reliable determination was at least 3 ng/ml plasma. The reproducibility of the method was satisfactory. The procedure can be used effectively to quantitate nifedipine in the human plasma.https://daru.tums.ac.ir/index.php/daru/article/view/85https://daru.tums.ac.ir/index.php/daru/article/download/85/85

DARU Journal of Pharmaceutical Sciences74FLAVONOID GLYCOSIDES FROM LEDUMPALUSTRE L. SUBSP. DECUMBENS (AIT) HULTON58Changdong JinWendy StrembiskiYuliya KulchytskaRonald G. Micetich Mohsen Daneshtalab20151006From the leaves of Ledum palustre L. subsp. decumbens (Ait.) Hulton a novel flavonoid glyco¬side, quercetin 3-p-D-(6-^-coumaroyI) galactoside, and quercetin 3-p-D-(6-/?-hydroxy-benzoyI)galactoside, were isolated. Their structures were elucidated by spectroscopic methods. Bothcompounds showed in vitro antifungal activity against Cryptococcus moformcms, Saccharomycescerevisiae, Aspergillus niger and Candida albicans, as well as cytotoxicity against KB cells.https://daru.tums.ac.ir/index.php/daru/article/view/86https://daru.tums.ac.ir/index.php/daru/article/download/86/86

DARU Journal of Pharmaceutical Sciences74SCREENING OF 6-PYRUVOYL-TETRAHYDROPTERIN SYNTHASE ACTIVITY DEFICIENCY AMONG HYPERP HENYLALANINEMIC PATIENTS911DURDI QUJEQ20151006A deficiency of the phenylalanine hydroxylase activity or its cofactor tetrahydrobiopterin maylead to hyperphenylalamnemia and as a result, loss of IQ, poor school performance, andbehavior problems occurs. Deficiency in 6-pyruvoyl-tetrahydropterin synthase activity is themajor cause of tetrahydrobiopterin deficient phenylketonuria. In this study, blood specimensfrom 165 healthy volunteers and 127 children with phenylketonuria were used to determinethe 6-pyruvoyl-tetrahydropterin synthase activity. It was found that the activity of 6-pyruvoyl- tetrahydropterin synthase was decreased in comparison with control [23.46 +/-2.94, (mean +/- SD, mmol/ ml/h, n=I27) vs. 127.63 +/- 4.52, n=165), p<0.05]. Results ofthis study indicate that examination of 6-pyruvoyl-tetrahydropterin synthase activity is helpfuland may lead to the diagnosis cause of hyperphenylalaninemia.https://daru.tums.ac.ir/index.php/daru/article/view/87https://daru.tums.ac.ir/index.php/daru/article/download/87/87

DARU Journal of Pharmaceutical Sciences74VOLATILE CONSTITUENTS OF THE FRUIT OF CORIANDRUM SATIVUML. FROM ISFAHAN1214ALIREZA GHANNADIDAVOOD SADEH20151006The essential oil of the fruits of Coriandrum sativum L. which is cultivated in Isfahan was isolated by steam distillation and analyzed by TLC, GC, GC/MS and ^-NMR. Eight compounds representing 95.3% of the total components were characterized. Linalool (56.2%), y- terpinene (12.0%) and 5-3-carene (9.7%) were the major constituents of the oil which were obtained in 0.68% (V/W) yield.https://daru.tums.ac.ir/index.php/daru/article/view/88https://daru.tums.ac.ir/index.php/daru/article/download/88/88

DARU Journal of Pharmaceutical Sciences74COMPARATIVE BIOAVAILABILITY OF FOUR ORAL FORMULATION OF CEPHRADINE CAPSULES1518MOHAMMAD K. HASSANZADEHSEDIGHEH FAZLI-BAZZAZ SHAHRAM AFZALI20151006A crossover study was utilised to compare the bioavailability of four different brands of cephradine capsules in eight normal human volunteers. Relative bioavailability of three local generic dosage forms was compared with a commercial cephradine capsule. The plasma and urine cephradine concentrations were determined by a microbiological assay (disk diffusion) using Staphylococcus aureus (ATCC 29737) as the test organism. Relative bioavailability and pharmacokinetic parameters of local generic dosage forms of cephradine were calculated using the plasma and urine data. Statistical analysis of the data indicated no significant differences (P=0.05) between brands of cephradine capsules. Results of this study showed that the extent and rate of absorption of various tested capsules are comparable and all tested brands are compatible and bioequivalent.https://daru.tums.ac.ir/index.php/daru/article/view/89https://daru.tums.ac.ir/index.php/daru/article/download/89/89

DARU Journal of Pharmaceutical Sciences74DYS-REGULATION OF EXTRACELLULAR MATRIX PROTEINS TURNOVER BY HIGH GLUCOSE CONCENTRATIONS IN CULTURED HUMAN GLOMERULAR MESANGIAL CELLS1924ROHOLLAH HOSSEINIGERHARD HAMPEL KLAUS JUNG20151006Diabetic glomerulopathy is generally believed to be the major cause for the development of chronic renal failure in diabetes mellitus. Diabetic glomerulosclerosis is characterized by the accumulation of extracellular matrix proteins such as fibronectin and type IV collagen in the mesangium. In this study, the effect of high glucose (33.6 mM) on fibronectin and type IV collagen concentrations in the supernatant of human glomerular mesangial cell culture was studied. The concentrations of fibronection and type IV collagen in tissue culture supernatant, assayed by ELISA techniques increased significantly (P<0.01) by the high glucose level (33.6 mM) after 6 days incubation. Direct cell counting and thymidine incorporation methods showed that high glucose concentrations (33.6 mM, 56 mM and 112 mM) inhibit the mesangial cell (MC) proliferation in concentration-dependent manner. To study the osmotic effect of high glucose concentrations, the mesangial cells were also cultured in the presence of manitol and it was found that manitol did not have effect on cellular proliferation but increased fibronectin and type IV collagen concentrations significantly (P<0.05) in the supernatants. These results indicate that the increase of synthesis and/or decrease degradation of fibronectin and type IV collagen by MCs may, in part, result from changes in osmolarity induced by high glucose concentration. These results suggest that elevation of fibronectin and type IV collagen production and/or decrease their degradation by the mesangial cells may play an important role in the accumulation of these extracellular matrix proteins which is common to diabetic glomerulosclerosis.https://daru.tums.ac.ir/index.php/daru/article/view/90https://daru.tums.ac.ir/index.php/daru/article/download/90/90

DARU Journal of Pharmaceutical Sciences74CONSTITUENTS OF THE ESSENTIAL OIL OF CHIMONANTHUS ERA GRANCE LINDLE2526KATAYOUN JAVIDN1ARAMTN MIRIMARYAM CHERIKI ABBAS SHAFIEE20151006The constituent of the essential oil ofChimonanthus fragrance Lindle (Calycanthaceae) were characterized by GLC and GC/MS. Twenty components representing 97% of the oil composition of which fifteen were sesquiterpenes were identified. The major components were p-Elemene, p-Caryophyllene, y-Cadinene, y-Bisabolene, p-EIemenone and a-Eudesmol.https://daru.tums.ac.ir/index.php/daru/article/view/91https://daru.tums.ac.ir/index.php/daru/article/download/91/91

DARU Journal of Pharmaceutical Sciences74CHEMICAL CONSTITUENTS OF THE ESSENTIAL OIL OF THYMUS KOTSCHYANUS BOISS. & HOHEN. FROM IRAN2728ABDOLHOSSEIN RUSTAIYANTARANEH LAJEVARDIMOHAMMAD RABBANIMOHAMMAD YARI SHIVA MASOUDI20151006Water-distilled essential oil of Thymus kotschyarms Boiss<& Hohen. was examined by GC and GC/MS. Fifteen constituents representing 80.7% of the oil were characterized of which thymol (38.0%), carvacrol (14.2%) and 1,8- cineole (13.2%) were the main compounds which were identified.https://daru.tums.ac.ir/index.php/daru/article/view/92https://daru.tums.ac.ir/index.php/daru/article/download/92/92