DARU Journal of Pharmaceutical Sciences71Evaluation of antinociceptive and antimicrobial activities of galbanum plant (Ferula gumosa Boiss)122B. S. Fazly BazzazH. Parsaei+G. Haririzadeh A. N. Shoshtari20151006To evaluate the antinociceptive and antimicrobial activities of galbanum plant (Ferula gumosa), various parts of the plant were collected at specific seasons. Aerial parts and root of the plant were dried in shady place and grinded to desirable. Unnatural and natural gum resins did not have the drying and grinding stages. The alcohol-aqueous (33%)extract was obtained by masuration and the solvent was removed by rotary evaporator at low temperature and vaccum condition. The essential oil was extracted by water and steam distillation. Its antinociceptive effect was investigated in mice using hot plate method. Antibacterial effect was determined using paper disk method. The results suggest that the maximum antinociceptive effect (efficacy) of root and aerial parts extract was higher than morphine and maximum effect of unnatural and natural gum resins extract was equal to morphine. The maximum effect of essential oil and unnatural gum resin was less than morphine but potency of these preparations were less than morphine. The amount of microbial growth inhibition of all extracts was less than chloramphenicol (30 ;ug) on gram positive bacteria, but these extracts have not any growth inhibitory effect on gram negative baceria. These extracts inhibited fungus growth equal to nystatin (100units). These results in conjunction with economic considerations suggest the usefulness of aerial parts of plant for medical treatment.https://daru.tums.ac.ir/index.php/daru/article/view/62https://daru.tums.ac.ir/index.php/daru/article/download/62/62DARU Journal of Pharmaceutical Sciences71Phytochemical Analysis of Essential Oil of Citrus bigardia L Growing in Shiraz2328N. GhassemiM. AzadbakhtSh Sabzevari20151006The comopition of the essential oil from the blossoms of citrus bigardia growing in shiraz region of Iran was investigated by GC and GC/Ms. Ten monoterpenes and two sesquiterpenes were among the main constituents in the oil.The main components of the volatile oil were B - pinene (4.07%), limonene (0.70%), terpinenyl acetate (4. 92% , linaloo! (38.3%), - terpinyl acetate (8.15%), graniol (4.39%), iinalyl acetate (10.24%) mytcene (4.08%), neryl acetate (4.72%) and farnesyi acetate (4.35%). Among these components the predominant ones were linalool and linalyl acetate. The presence of both predominant components were confirmed by TLC and GC.https://daru.tums.ac.ir/index.php/daru/article/view/63https://daru.tums.ac.ir/index.php/daru/article/download/63/63DARU Journal of Pharmaceutical Sciences71A New Spectorphotometric Procedure for the determination of Cyanide2938F. ShamsaZ. Hemmaceyan-Ettifak20151006A new spectrophotometric procedure forthe determination of cyanide is reported. The principle of the procedure is the bleaching of the cobalt - 0"- nitrozo-R - naphthol complex. The oragne colored complex between the cobalt and the reagent is formed in an alkaline pH and is extractabie by chloroform. Beer.s law is obeyed at 4-24 ug sodium cyanide. The effect of various cations and anions on the analytical procedure is also reported.https://daru.tums.ac.ir/index.php/daru/article/view/64https://daru.tums.ac.ir/index.php/daru/article/download/64/64DARU Journal of Pharmaceutical Sciences71Preliminary study of polysaccharides in the tragacanth of Astragalus gossipinus Fisch and Astragalus keyserlingii Bunge3954H. Ebrahimzadeh F. Mighani20151006From the point of gum production, Fabaceae is one of the most richest plant families. Tragacanth is one of the most important gums and has medicianl, industrial and food applications. The soluble and insoloble fractions are 40 and 60% in white gum and 70 and 30% in yellow gum, respectively. These fractions do not show considerable seasonal variations. Total sugar in white gum and yellow gum are 70% and 90% , respectively; monosaccharides in both kind of tragacanths include galacturonic acid, galactose, glucose, arabinose, xylose, fucose, and rhamnose and the amount of xylose in the composition is higher than that of others. Insoluble fraction of white gum, the amount of xylose is more than that of arabinose pJus fucose while in yellow gum its amount is lower than that of arabinose plus fucose. fln insoluble fraction of both kind of tragacanths, the amount of arabinose plus fucose is high. The quality of white gum, in comparison to yellow gum, is better due to the higher in soluble fraction and arabinose plus fucose to xylose in this fraction . The gum tragacartti of both species has a xylan backbone.https://daru.tums.ac.ir/index.php/daru/article/view/65https://daru.tums.ac.ir/index.php/daru/article/download/65/65DARU Journal of Pharmaceutical Sciences71Pharmacognostical Investigations on Sambucus ebulus L. and Sambucus nigra L5565Dr. A. R. GhannadiDr. N. Ghassemi-Dehkordi20151006Several species of the genus Sambucus have been used in treating symptoms of the common cold and some skin ailments. Four species of this plant are growing extensively in Iran. One of these species is S.ebulus. In this study, S. ebulus was examined botanically and phytochemicatly in comparison to S. nigra.Morphological as well as microscopical characteristics of S. ebulus and S. nigra were examined. By means of TLC in comparison to authentic samples, Rutin, Hyperin. Apigenin and Chlorogenic acid were identified in these plants. By preparative TLC method, Rutin is isolated and then purified from these plants. The structure of Rutin was determined by the UV-Vis. Techniques in methanol and by addition of the shift reagents and hydrolysis. The quantitative determinations of fiavonoids in these plants were also performed by using an UV-Vis. Spectroscopy method.https://daru.tums.ac.ir/index.php/daru/article/view/66https://daru.tums.ac.ir/index.php/daru/article/download/66/66DARU Journal of Pharmaceutical Sciences71Effect of Urea Fertilizer on the Weight of Different Parts of Datura stramonium and their Alkaloidal Contents at Different Developmental Stages6672S. AfsharypuorA. MostajeranM. R Shaneh-Saz20151006Plants of Datura Stramonium var. stramonium were cultivated in pots at different nitrogen levels (60,120, 180, 240, and 300 kg/ha) using urea fertilizer as the nitrogen source. At various developmental stages, the weights of different parts of the plants which recieved 60-240 kg N/ha were significantly increased as compared with the relevant parts of untreated (control) plants. The 240 kg N/ha usually caused the highest increases in the weights of different parts of the plant.Atropine and scopolamine contents of each dried part of the plant were determined by a previously published TLC-spectrophotometric method. The stem of 80 days old pretreated plants with 180 kg N/ha accumulated significantly the highest percentages of atropine and scopolamine. However, the 120 kg N/ha level caused the highest enhancement in the yield of atropine (65.24 %) and scopolamine (40.38%) in the whole plant during the reproductive (80 days old) stage as compared with the yield of these alkaloids in the relevant controls.https://daru.tums.ac.ir/index.php/daru/article/view/67https://daru.tums.ac.ir/index.php/daru/article/download/67/67DARU Journal of Pharmaceutical Sciences71Pharmacokinetic Study of Ketoprofen After Oral Administration of Sustained Release and Non-Sustained Release Dosage Forms7383Mohammad. K. HassanzadehA.H. Beckett20151006Six healthy male subjects involved in a crossover bioavailability study to compare the pharmacokinetics of ketoprofen after single oral administration of the drug (1 OOmg) as non sustained release or sustained release pellets dosage forms. A specific and sensitive high performance liquid chromatographic assay procedure was used to analyse the plasma and urine samples. The absorption from sustained release pellets dosage form was slower and more sustained than from non sustained release capsules, but almost complete. The bioavailability properties of this sustained release pellets dosage form of ketoprofen appears suitable for clinical use with reduced frequency of daily dosing. KEY WORDS: Pharmacokinetics, Sustianed Release, Ketoprofen, In-vivohttps://daru.tums.ac.ir/index.php/daru/article/view/68https://daru.tums.ac.ir/index.php/daru/article/download/68/68