DARU Journal of Pharmaceutical Sciences181Selection for autochthonous bifidobacteial isolates adapted to simulated gastrointestinal fluid.5766HJamalifarBBigdeliJNowrooziH SZolfaghariM RFazeli20151109Bifidobacterial strains are excessively sensitive to acidic conditions and this can affect their living ability in the stomach and fermented foods, and as a result, restrict their use as live probiotic cultures. The aim of the present study was to obtain bifidobacterial isolates with augmented tolerance to simulated gastrointestinal condition using cross-protection method.Individual bifidobacterial strains were treated in acidic environment and also in media containing bile salts and NaCl. Viability of the acid and acid-bile-NaCl tolerant isolates was further examined in simulated gastric and small intestine by subsequent incubation of the probiotic bacteria in the corresponding media for 120 min. Antipathogenic activities of the adapted isolates were compared with those of the original strains.The acid and acid-bile-NaCl adapted isolates showed improved viabilities significantly (phttps://daru.tums.ac.ir/index.php/daru/article/view/489