DARU Journal of Pharmaceutical Sciences144Spasmolytic effect of Vitis vinifera leaf extract on rat colon203207Gharib Naseri M.K.Zarei M.Amiri O.20151006Vitis vinifera (grape) leaf has been used traditionally to treat diarrhea and its extract induces relaxation in rat aorta and uterus. The aim of present study was to investigate the effect of grape leaf hydroalcoholic extract (GLHE) on rat colon contractions induced by some spasmogens. A piece of distal colon from male adult Wistar rats were dissected and mounted in an organ bath containing Tyrode solution and colon contractions recorded by an isotonic transducer under 1g resting tension. The GLHE (0.5- 4 mg/ml) reduced the contractions induced by KCl (60 mM), BaCl2 (4 mM), acetylcholine (1 μM) dose-dependently (Phttps://daru.tums.ac.ir/index.php/daru/article/view/290https://daru.tums.ac.ir/index.php/daru/article/download/290/290